Cryopreservationin marine fish
Keywords:
Cryopreservation, FishAbstract
This document reviews the knowledge developed to date on the theory and techniques of marine fish cryopreservation. Cryopreservation has established itself as an effective tool for conserving the genetic material of various species with commercial or ecological relevance, or those facing extinction problems. The effectiveness of the protocol can be determined by monitoring the quality and viability of samples preserved under cryogenic conditions. It is suggested that the responsible use and marketing of cryopreserved semen be explored, and that the development of cryopreservation techniques be promoted, with the goal of expanding the tools available for use in aquaculture farms.
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Babiak, I., Bolla, S., & Ottesen, O. (2008). Suitable methods for cryopreservation of semen from Atlantic halibut (z). Aquaculture International, 16, 561–572.Betsy, J., & Kumar, S. (2020). Cryopreservation: History and development. Cryopreservation of fish gametes, 135-149.
Betsy, J., & Kumar, S. (2020). Cryopreservation: History and development. Cryopreservation of fish gametes, 135-149.
Bobe, J., & Labbé, C. (2008). -Chilled storage of sperm and eggs. In Methods in Reproductive Aquaculture (pp. 241-258). CRC Press.
Boryshpolets, S., Kholodnyy, V., Cosson, J., & Dzyuba, B. (2020). Fish sperm quality evaluation after cryopreservation. Cryopreservation of fish gametes, 117-133.
Bustamante-González, J. D., Rodríguez-Gutiérrez, M., Cortés-García, A., Arenas-Ríos, E., Figueroa-Lucero, G., & Ávalos-Rodríguez, A. (2019). Fisiología y criopreservación del espermatozoide en teleósteos. AquaTIC, 53, 1-17.
Cabrita, E., Robles, V., Cuñado, S., Wallace, J. C., Sarasquete, C., & Herráez, M. P. (2005). Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 mL macrotubes. Cryobiology, 50(3), 273–284.
Cabrita, E., Engrola, S., Conceição, L. E. C., Pousão-Ferreira, P., & Dinis, M. T. (2009). Successful cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus. Aquaculture, 287, 152–157.
Cabrita. E., Sarasquete C., Martınez-Paramo S., Robles V., Beirao J., Perez-Cerezale S. & Herraez M. P. (2010). Cryopreservation of fish sperm: applications and perspectives. J. Appl. Ichthyol. 26, 623–635.
Cabrita, E., Horváth, Á., Marinović, Z., & Asturiano, J. F. (2022). Technologies and strategies for ex situ conservation of aquatic organisms: the role of cryopreservation in long-term management. In Cellular and Molecular Approaches in Fish Biology (pp. 1-48). Academic Press.
Chen, Y. K., Liu, Q. H., Li, J., Xiao, Z. Z., Xu, S. H., & Shi, X. H. (2010). Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red sea bream (Pagrus major) sperm. Cryobiology, 61(2), 189–193.
Chereguini, O., De La Banda, I. G., Herrera, M., Martinez, C. and De La Hera, M. (2003). Cryopreservation of Turbot Scophthalmus maximus (L.) sperm: fertilization and hatching rates. Aquaculture Research, 34(9), 739-747.
Cuevas‐Uribe, R., Chesney, E. J., Daly, J. and Tiersch, T. R. (2015). Vitrification of sperm from marine fish: effect on motility and membrane integrity. Aquaculture Research, 46(7), 1770-1784.
Ciereszko, A., Judycka, S., Nynca, J., Słowińska, M., & Dietrich, M. A. (2020). Factors influencing milt quality in fishes and its usefulness to cryopreservation. Cryopreservation of fish gametes, 25-67.
Franěk, R., & Pšenička, M. (2020). Cryopreservation of germ stem cells in fish. Cryopreservation of Fish Gametes, 285-312.
Gaitán-Espitia, J. D., Martínez-Silva, M. G., Borrero, C., Ramírez, L. and Valencia, J. M. B. (2013). Cryogenic preservation of sperm from lane snapper (Lutjanus synagris): Testing the effects of extenders and freezing rates on sperm quality. Aquaculture, 384-387, 6-12.
Gallego, V., & Asturiano, J. F. (2019). Fish sperm motility assessment as a tool for aquaculture research: a historical approach. Reviews in Aquaculture, 11(3), 697-724.
Gwo, H.-H., Weng, T.-S., Fan, L.-S., & Lee, Y.-H. (2005). Development of cryopreservation procedures for semen of Pacific bluefin tuna Thunnus orientalis. Aquaculture, 249, 205–211.
Hagedorn, M. M., Daly, J. P., Carter, V. L., Cole, K. S., Jaafar, Z., Lager, C. V., & Parenti, L. R. (2018). Cryopreservation of fish spermatogonial cells: the future of natural history collections. Scientific Reports, 8(1), 6149.
He, Q., Zhao, E., Lu, Y., Yan, M., Huang, C. and Dong, Q. (2012). Evaluation of activation and storage conditions for sperm of yellow drum Nibea albiflora. Aquaculture, 324, 319-322.
Hezavehei, M., Sharafi, M., Kouchesfahani, H. M., Henkel, R., Agarwal, A., Esmaeili, V., & Shahverdi, A. (2018). Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reproductive biomedicine online, 37(3), 327-339.
Horokhovatskyi Y., Sampels S., Cosson J (2016). Lipid composition in common carp (Cyprinus carpio) sperm possessing different cryoresistance. Cryobiology 73:282–285.
Ji, X., Chen, S., Tian, Y., Yu, G., Sha, Z., Xu, M. and Zhang, S. (2004). Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization. Aquaculture, 241(1-4), 517-528.
Jiang, J., Yan, J., Zhu, J. and Yang, W. (2011). Sperm cryopreservation and the cytoarchitecture damage detection of Pseudosciaena crocea. Journal of Agricultural Biotechnology, 19(4), 725-733.
Judycka, S., Nynca, J., Liszewska, E., Mostek, A., & Ciereszko, A. (2019). Comparative analysis of sperm freezability of sex-reversed female brook trout and sex-reversed female rainbow trout semen. Aquaculture, 498, 201-207.
Koh, I. C. C., Yokoi, K. I., Tsuji, M., Tsuchihashi, Y., & Ohta, H. (2010). Cryopreservation of sperm from seven-band grouper, Epinephelus septemfasciatus. Cryobiology, 61(3), 263–267.
Lim, H. K., & Le, M. H. (2013). Evaluation of extenders and cryoprotectants on motility and morphology of longtooth grouper (Epinephelus bruneus) sperm. Theriogenology, 79(5), 867–871.
Magnotti C, Cerqueira V, Lee-Estevez M, Farias J, Valdebenito I, Figueroa E (2016) Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species. Reviews in Aquaculture pp. 1–11.
Magnotti, C., Cerqueira, V. R., Lee-Estevez, M., Farías, J. G., Valdebenito, I., & Figueroa, E. (2018). Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species. Reviews in Aquaculture, 10(1), 15-25.
Martínez-Páramo, S., Pérez-Cerezales, S., Gómez-Romano, F., Blanco, G., Sánchez, J. A., & Herráez, M. P. (2009). Cryobanking as tool for conservation of biodiversity: effect of brown trout sperm cryopreservation on the male genetic potential. Theriogenology, 71(4), 594-604.
Martínez-Páramo, S., Horváth, Á., Labbé, C., Zhang, T., Robles, V., Herráez, P., & Cabrita, E. (2017). Cryobanking of aquatic species. Aquaculture, 472, 156-177.
Medina-Robles, V. M., Duarte-Trujillo, A. S., & Cruz-Casallas, P. E. (2020). Crioconservación seminal en peces de agua dulce: aspectos biotecnológicos, celulares y bioquímicos. Orinoquia, 24(2), 51-78.
Paniagua-Chávez, C. G., Ortiz-Gallarza, S. M., & Aguilar-Juárez, M. (2011). Subsistema Nacional de Recursos Genéticos Acuáticos: uso de la criopreservación para la conservación de los recursos genéticos acuáticos en México. Hidrobiológica, 21(3), 415-429.
Peatpisut, T., & Bart, A. N. (2010). Cryopreservation of sperm from natural and sex-reversed orange-spotted grouper (Epinephelus coioides). Aquaculture Research, 42(1), 22–30.
Riesco, M. F., Valcarce, D. G., & Robles, V. (2022). Molecular approaches on DNA damage evaluation after primordial germ cell cryopreservation in zebrafish. In Cellular and Molecular Approaches in Fish Biology (pp. 49-68). Academic Press.
Riley, K. L. P. (2002). Refrigerated storage and cryopreservation of sperm for the production of red snapper and snapper hybrids. Louisiana State University and Agricultural and Mechanical College. 73.
Riley, K. L., Holladay, C. G., Chesney, E. J. and Tiersch, T. R. (2004). Cryopreservation of sperm of red snapper (Lutjanus campechanus). Aquaculture, 238(1-4), 183-194.
Thilak Pon Jawahar, K.& Betsy, J. (2020). Cryopreservation of Fish Gametes: An Overview. In: Betsy, J., Kumar, S. (eds) Cryopreservation of Fish Gametes. Springer, Singapore.
Trigo, P., Merino, O., Figueroa, E., Valdebenito, I., Sánchez, R., & Risopatrón, J. (2015). Effect of short‐term semen storage in salmon (O ncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry. Andrologia, 47(4), 407-411.
Sanches, E., Oliveira, I., Serralheiro, P., & Cerqueira, V. (2013). Cryopreservation of mutton snapper (Lutjanus analis) sperm. Annals of the Brazilian Academy of Sciences, 85(3), 1083–1091.
Sanches, E., Oliveira, I., Serralheiro, P., & Cerqueira, V. (2015). Sperm cryopreservation of lane snapper Lutjanus synagris (Linnaeus, 1758). Brazilian Journal of Biology.
Vuthiphandchai, V., Thadsri, I. and Nimrat, S. (2009). Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296(1-2), 58-64.
Yongsheng, T., Jingjing, Z., Zhentong, L., Ziqi, L., & Linna, W. (2020). Cryopreservation of Marine Fish Sperm. Cryopreservation of Fish Gametes, 187-210.

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