Prevalence of anti-Salmonella enterica serovar Typhi antibodies in individuals from Culiacan, Sinaloa, Mexico

Authors

Keywords:

Antigen, Typhoid Fever, Seroprevalence, Immunodominant antigen

Abstract

To estimate the prevalence of specific antibodies against Salmonella enterica serovar Typhi (S. Typhi) in individuals from Culiacan, Sinaloa, Mexico. A cross-sectional study was conducted at the University Clinical Analysis Laboratory "MC Héctor Cuén Ojeda" in Culiacán, Sinaloa, Mexico, during the period 2009-2010. In this study, 366 individuals attended the laboratory to obtain a medical certificate. The serum of each individual was obtained and analyzed by Widal agglutination. Positive samples to Widal (dilution 1:160), were tested by Western blotting using an antigenic extract of S. Typhi (AEST). Of 366 sera analyzed, 10.11% (n=37) were Widal-positive. The Western blot developed using these sera revealed four prominent antigens: a 25 kDa protein was recognized by 35 sera (94.6%), 24 kDa and 30 kDa proteins were reactive to the same number of sera, with 34 (91.9%), and 33 (89.2%) recognized a 60 kDa protein. In conclusion, the anti-S. Typhi seroprevalence obtained was 10.11%, and four proteins derived from AEST were identified with high immunoreactivity (>89%), the 25 kDa antigen was the immunodominant. It is necessary to continue studying these detected antigens and increase our knowledge about the estimation of seroprevalence of S. Typhi, whose infection keeps Sinaloa within the 5 states with the highest incidence of Typhoid Fever in Mexico.

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Author Biographies

  • López-Moreno H. M., Facultad de Ciencias Químico Biológicas-UAS, México.

    Ph.D. López-Moreno has a degree in Chemist, Pharmaceutical Biology from the Faculty of Chemical-Biological Sciences (FCQB) of the Autonomous University of Sinaloa, and a Doctor of Science from the Direct Doctorate in Biomedical Sciences, UNAM. He is a “C” Senior Research Professor assigned to the FCQB-UAS since 2004. Member of the National System of Researchers (Level I); Name of QFB Generation 2012-2017; Evaluator of the Mexican Council for the Accreditation of Pharmaceutical Education (COMAEF) AC, from October 2010 to date; International Evaluator of the National Council for University Evaluation and Accreditation of Panama (CONEAUPA), from 2012 to date; PRODEP evaluator, SEP; CONAHCYT Project Evaluator; PROFAPI Project Evaluator, UAS; Technical Manager of 17 research projects (9 with external financing to the UAS); Peer reviewer of the journal “Vector Borne and Zoonotic Diseases”, the journal “Frontiers in Immunology” and the journal Biomedica of Colombia; Leader of the CAC Biomedical Biotechnology UAS-264; He cultivates two Lines of research: 1. Biomedical Biotechnology and 2. Apoptosis of Giardia lamblia; University Counselor of the UAS, from 2009-2010; Career Manager at QFB in 2008 and 2011; Honorary Member of the Sinaloense System of Researchers and Technologists; PRODEP Profile, SEP. He has supervised 55 Theses (4 Doctorates, 17 Master's, and 34 Bachelor's). He has published 38 Scientific Articles indexed in JCR, 18 Memoirs in Extenso, 13 Dissemination Publications, and 60 Presentations at National and International Congresses. He has 330 scientific citations.

     

  • Casal-Rodriguez K. A. , Laboratorio de Biomedicina Molecular, México.

    QFB and Master's Thesis in Biomedical Sciences

  • Beltrán-López E., Laboratorio de Biomedicina Molecular-UAS, México.

    Master in Forensic Sciences and Doctoral Thesis in Biomedical Sciences

  • Osuna-Ramirez I., Unidad de Investigación en Salud Pública, Louis Pasteur, UAS. México.

    PhD of Sciences with a specialty in Biostatistics

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Prevalence of anti-Salmonella enterica serovar Typhi antibodies In

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Published

2024-03-01

Issue

No. 1 (2024): QUIBIOUAS Revista de Ciencias Químico Biológicas

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Scientific articles

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How to Cite

LÓPEZ MORENO , H. S. ., Casal Rodríguez, K. A., Beltrán López, E., & Osuna Ramírez, I. (2024). Prevalence of anti-Salmonella enterica serovar Typhi antibodies in individuals from Culiacan, Sinaloa, Mexico. QUIBIOUAS Revista De Ciencias Químico Biológicas, 1, 33-39. https://revistas.uas.edu.mx/index.php/QBU/article/view/334