Enterococcus faecium isolated from the intestinal contents of Penaeus vannamei: safety, technological, and probiotic properties

Authors

Keywords:

Shrimp, Enterococcus, Microbiota, Probiotic

Abstract

The gut microbiota of shrimp has been recognized as a source of probiotic bacteria useful for biotechnological application. The aim of this study was to evaluate the survival and probiotic activity in vitro of  five lactic acid strains isolated from Penaeus vannamei. The strains were isolated from the shrimp gut and analyzed by sequencing the 16S rDNA gene. The survival of the strains was evaluated by challenges of acidity (pH 3, 5, 6 and 7), temperature (4, 15, 30, 37 and 45 °C) and salinity (4%, 6% and 8% NaCl) for 12 h. The technological, probiotic and safety capacity of the strains were determined by various in vitro tests. The five native strains corresponded to the order Lactobacillales and were phylogenetically related (>99.9%) to the E. faecium strain (DSM20477). Multivariate analysis indicated a similar survival rate (≈ 94%) among E. faecium strains, whose proliferation occurs at conditions >4 °C, pH 5 - 7 and <6%NaCl. Probiotic capacity varied among E. faecium strains (P <0.05) according to hydrophobicity, self-aggregation, co-aggregation and antagonistic properties. The technological potential of the strains was similar according to their acidifying, proteolytic and lipolytic power (P >0.05). Sensitivity to seven antibiotics and absence of hemolysis were also observed in all strains. The physiological characteristics of autochthonous strains of shrimp gut allow them to be proposed as potential probiotic agents for the development of biotechnological products.

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Author Biographies

  • Castañeda-Ruelas G. M., Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, México.

    A Full-time Professor and Researcher at the Faculty of Chemical-Biological Sciences of the Universidad Autónoma de Sinaloa, where teaching courses in the Bachelor of Science in Pharmaceutical and Biological Chemistry (FCQB-UAS). Member of the National System of Researchers, Level I, and recognized as an Honorary Researcher by Sinaloa System of Researchers and Technologists. Professional experience includes collaboration in scientific and technological research projects and the training of high-quality human resources at the Bachelor's, Master's in Science, and PHD in Science levels. Research findings have been published in indexed and peer-reviewed journals, book chapters, and national and international conferences.

  • Gámez-Bayardo S., Instituto de Capacitación y Educación Profesional  en Colima, México

    An accomplished professional with diverse experience in microbiological research, socio-formative teaching and learning, and languages. He currently serves as the Academic Deputy Director at the Institute of Training and Professional Education in Colima, where he focuses on planning, organizing, executing, and controlling cross-cutting projects to ensure academic excellence. In addition, he facilitates faculty induction courses and prepares course programming tailored to cutting-edge professional profiles.

  • Domínguez-Lagunes S., Universidad Autónoma de Sinaloa, México.

    He studied for a degree in "Pharmaceutical Biology" at the University of Los Mochis and completed his professional practices at the "Microbiological Research and Diagnostic Laboratory (LIDiM)" of the "Autonomous University of Sinaloa." During this time, he had the opportunity to present his thesis project titled "SURVIVAL FACTORS OF Vibrio parahaemolyticus ISOLATED FROM COSTA AZUL, ANGOSTURA" at various conferences in poster format. In 2019, he joined the Conacyt program for a Master's in Biotechnology with an Environmental focus, where he conducted his thesis project "Isolation and Molecular and Phenotypic Characterization of Native Bacteria with Probiotic Potential in Penaeus vannamei," from which the data for this article were obtained.

  • Fierros-Pérez C. E., Facultad de Biología, Autónoma de Sinaloa, México.

    Full-Time Tenured Professor and Researcher “B” at the Faculty of Biology of the Autonomous University of Sinaloa, with a Master's in Science specializing in Microbiology and a Doctorate in Educational Sciences focused on Research. She is part of the faculty teaching courses in Research Project Development, Research Seminar, and Thesis Seminars 2 and 3. She has been a tutor for students in both the Bachelor's in Biology and the Bachelor's in Biomedicine since 2015, and is a certified advisor for Social Service. She has served as an advisor and reviewer for undergraduate theses in the Faculty of Biology. Her research areas include Environmental Microbiology and Food Microbiology

  • Durán-Pérez S. A., Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, México.

    He graduated as QFB from the Faculty of Chemical-Biological Sciences, Autonomous University of Sinaloa, in August 2008. He completed master's studies in Biomedical Sciences and a doctorate in Health Biotechnology at the Faculty of Chemical-Biological Sciences of the Autonomous University of Sinaloa in October 2014 and March 2019, respectively. Research stay at the Experimental Medicine Unit (UME) of the National Autonomous University of Mexico in 2014 and 2016. He has been a member of the SNII since 2020 at level C and of the Mexican Bioinformatics Network since 2022. Fruitful collaborations have marked his research journey, as he has participated as a manager and collaborator in research projects funded by PROFAPI-UAS, and through collaboration agreements with local Mexican companies.
    His projects are focused on a wide range of diseases, studying the biology and etiology of infectious, bacterial, fungal, parasitic, and viral diseases of regional impact, such as trichomoniasis, giardiasis, and candidiasis, among others, as well as viral infections caused by papillomavirus, cytomegalovirus, rotavirus, and norovirus.
    He has published scientific and popular articles in specialized journals and presented the results of his research at national and international conferences.

  • Hernández-Díaz L. J., Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, México.

    Research Professor assigned to the Faculty of Chemical Biological Sciences of the Autonomous University of Sinaloa (UAS). Doctor of Sciences in the specialty Health Biotechnology (FCQB-UAS), 2016. He has published 4 articles in international peer-reviewed and indexed scientific journals. He has presented 21 research papers at conferences: 10 internationally and 11 nationally. Recent participation as a reviewer for the International Journal of Environmental Health Research. He completed a doctoral stay at the School of Medicine at Emory University, USA. He is part of the teaching staff of the chairs of four subjects (microbiology, mycology, food safety and cellular biology) of the Faculty of Chemical Biological Sciences, teaching practices in the different subjects of the area of ​​microbiology, advisor of 24 social service reports . Advisor and/or tutor of at least 10 bachelor's theses. Reviewer and/or grader member of around 15 bachelor's theses. Participation as a group tutor of students from 2018 to date and his lines of research are on epidemiology and molecular biology of microbiological pathogens.

  • Jiménez-Edeza M. (Autor de correspondencia), Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, México

    Full-time Professor and Researcher, affiliated to the School of Chemical and Biological Sciences at the Universidad Autónoma de Sinaloa, where she teaches courses at the Bachelor degrees programs of Chemical Pharmaceutical Biology and Biochemical Engineering; as well as the Integral Posgraduate Program in Biotechnology. Member of the National System of Researchers level I and Honorary Researcher of the Sinaloa System of Researchers and Technologists. She is founder and responsible for the Microbiological Research and Diagnostic Laboratory (LIDiM) of the FCQB-UAS, where she annually directs the Training of High-Quality Human Resources in Bachelor, Master of Science and Doctorate in Science. Her research experience is related to the identification and characterization of foodborne microorganisms, as well as their presence and distribution in water and environment. Her research results have been published in indexed and refereed journals and book chapters. In addition, she actively participates in national and international conferences. Her professional experience includes collaboration in the implementation of productive projects with the health, education and business sectors.

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Enterococcus faecium isolated from the intestinal contents of Penaeus vannamei: safety, technological, and probiotic properties

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2024-10-30

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No. 2 (2024): QUIBIOUAS Revista de Ciencias Químico Biológicas

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Castañeda Ruelas , G. M., Gámez Bayardo, S., Domínguez Lagunes , S., Fierros Pérez, C. E., Durán Pérez, S. A. ., Hernández Díaz , L. de J., & Jiménez Edeza , M. . (2024). Enterococcus faecium isolated from the intestinal contents of Penaeus vannamei: safety, technological, and probiotic properties. QUIBIOUAS Revista De Ciencias Químico Biológicas, 2, 35-46. https://revistas.uas.edu.mx/index.php/QBU/article/view/819